Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting

Table 4 Results of pathogen detection using RPM-Flu 3.1 microarray

Farm	Sample	Most likely ID*
1	B1	K. pneumoniae, P. stutzeri
	B2	K. pneumoniae, P. stutzeri
	B3	K. pneumoniae
	B4	no detection
	B5	no detection
	B11	no detection
	B12	K. pneumoniae, P. aeruginosa, Staph. (mecA gene)
	B13	K. pneumoniae, Pseudomonas
	B14	K. pneumoniae, P. aeruginosa, Staph. (mecA gene)
	B15	K. pneumoniae, P. putida, Staph. (mecA gene)
	B25	K. pneumoniae, Pseudomonas
	B26	K. pneumoniae
	B27	K. pneumoniae, P. putida
	B28	K. pneumoniae, P. aeruginosa, Staph. (mecA gene)
	B35	no detection
2	H2	no detection
	H3	no detection
	H4	(P. aeruginosa or M. catarrhalis)
6	HA5	(P. aeruginosa or M. catarrhalis)
	HA6	no detection
	HA7	Pseudomonas
5	A1	no detection
	A2	P. putida
	A16	no detection
9	J2	P. aeruginosa, E. sakazakii
	J7	no detection
	J10	no detection
4	W3	K. pneumoniae
	W4	P. aeruginosa
	W6	(Pseudomonas or Moraxella or Methylobacillus)

*Most likely ID was determined using CIBSI algorithm and based on similarity analysis of the sequences obtained from the microarray with sequences deposited GenBank at the time of conducting the analysis (April 2010). Abbreviations used: K. pneumoniae = Klebsiella pneumoniae, P. stutzeri = Pseudomonas stutzeri, Staph. = Staphylococcus spp., P. aeruginosa = Pseudomonas aeruginosa, P. putida = Pseudomonas putida, M. catarrhalis = Moraxella catarhalis, E. sakazakii = Enterobacter sakazakii. Organism names enclosed in parentheses denote result of hybridization of a single microarray tile, which cannot be unambiguously identified based on the obtained sequence.

ISSN: 1478-4505