Skip to main content

Advertisement

Table 4 Results of pathogen detection using RPM-Flu 3.1 microarray

From: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting

Farm Sample Most likely ID*
1 B1 K. pneumoniae, P. stutzeri
  B2 K. pneumoniae, P. stutzeri
  B3 K. pneumoniae
  B4 no detection
  B5 no detection
  B11 no detection
  B12 K. pneumoniae, P. aeruginosa, Staph. (mecA gene)
  B13 K. pneumoniae, Pseudomonas
  B14 K. pneumoniae, P. aeruginosa, Staph. (mecA gene)
  B15 K. pneumoniae, P. putida, Staph. (mecA gene)
  B25 K. pneumoniae, Pseudomonas
  B26 K. pneumoniae
  B27 K. pneumoniae, P. putida
  B28 K. pneumoniae, P. aeruginosa, Staph. (mecA gene)
  B35 no detection
2 H2 no detection
  H3 no detection
  H4 (P. aeruginosa or M. catarrhalis)
6 HA5 (P. aeruginosa or M. catarrhalis)
  HA6 no detection
  HA7 Pseudomonas
5 A1 no detection
  A2 P. putida
  A16 no detection
9 J2 P. aeruginosa, E. sakazakii
  J7 no detection
  J10 no detection
4 W3 K. pneumoniae
  W4 P. aeruginosa
  W6 (Pseudomonas or Moraxella or Methylobacillus)
  1. *Most likely ID was determined using CIBSI algorithm and based on similarity analysis of the sequences obtained from the microarray with sequences deposited GenBank at the time of conducting the analysis (April 2010). Abbreviations used: K. pneumoniae = Klebsiella pneumoniae, P. stutzeri = Pseudomonas stutzeri, Staph. = Staphylococcus spp., P. aeruginosa = Pseudomonas aeruginosa, P. putida = Pseudomonas putida, M. catarrhalis = Moraxella catarhalis, E. sakazakii = Enterobacter sakazakii. Organism names enclosed in parentheses denote result of hybridization of a single microarray tile, which cannot be unambiguously identified based on the obtained sequence.