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Table 4 Performance of culture in relation to smear microscopy (2010–2012)

From: Feasibility of establishing a biosafety level 3 tuberculosis culture laboratory of acceptable quality standards in a resource-limited setting: an experience from Uganda

Culture method

Performance indicator

2010 N (%)

2011 N (%)

2012 N (%)

LJ culture

Total (n)

3,818

5,186

1,486

 

Culture positive (MTBc)

69 (1.8)

683 (13.2)

447 (30.1)

 

Culture negative

3,749 (98.2)

4,503 (86.8)

1,039 (69.9)

 

Culture contaminated

70 (1.8)

287 (5.5)

48 (3.2)

 

Smear negative among culture positive

19 (27.54)

255 (37.3)

172 (38.5)

 

Smear positive among culture positive

46 (66.7)

377 (55.2)

264 (59.1)

 

Smear positive among culture contaminated

3 (4.3)

54 (18.8)

14 (29.2)

 

Smear positive among culture negative

21 (0.6)

66 (1.5)

72 (6.9)

MGIT culture

Total (n)

3,818

5,056

1,226

 

Culture positive M. tuberculosis (MTBc)

107(2.8)

777 (15.4)

352 (28.7)

 

Culture negative

3,285 (86.0)

2,705 (53.5)

682 (55.6)

 

Culture positive with MOTT

112 (2.9)

253 (5.0)

55 (4.5)

 

Culture contaminated

314 (8.2)

1,321 (26.1)

137 (11.2)

 

MOTT among smear positive

2 (2.8)

33 (6.6)

14 (6.6)

 

Smear negative among culture positive (MTBc)

56 (52.3)

575 (74.0)

211 (60.0)

 

Smear positive among culture positive (MTBc)

62 (57.9)

452 (58.2)

195 (55.4)

 

Smear positive among culture contaminated

0 (0.0)

23 (1.7)

5 (3.6)

 

Smear positive among culture negative

8 (0.2)

23 (0.8)

12 (1.7)

  1. Mycobacterial identifications on LJ were done basing on morphological features and Ziehl-Neelsen microscopy were done on doubtful colonies [15].
  2. LJ, Lowenstein-Jensen; MGIT, Mycobacterial growth indicator tube; MOTT, Mycobacterium other than tuberculosis; MTBc, Mycobacterium tuberculosis complex.