Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting

Table 3 Efficiency of detection of Influenza B RNA stored on FTA paper incubated at elevated temperatures ¹

Influenza B RNA concentration (copies/microliter)	Duration of incubation (hours)									Incubation temperatures (°C)
	24			72			240
	1	2	3	1	2	3	1	2	3
10⁶	+	+	+	+	+	+	+	+	+	20-25 (RT)
	+	+	+	+	+	+	+	+	+	30
	+	+	+	+	+	+	+	+	+	30-45 (outdoors)
10⁴	+	+	+	+	+	+	+	+	+	20-25 (RT)
	+	+	+	F	+	+	+	+	+	30
	+	+	+	+	+	+	+	+	+	30-45 (outdoors)
10²	−	−	−	+	+	F	−	F	F	20-25 (RT)
	−	−	−	F	F	−	−	−	−	30
	F	F	F	−	F	F	−	−	−	30-45 (outdoors)
10⁶ (control RNA)	+			+			+			−20 (freezer)
10² (control RNA)	+			+			+			−20 (freezer)

¹Each experiment for particular combination of sample concentration and temperature was run in triplicate. Plus sign indicates that RT-PCR detection produced strong band of expected size that was observed on a gel; F indicates a faint band and a minus sign indicates absence of an amplification product detectable by visual inspection on a gel.

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