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Table 3 Efficiency of detection of Influenza B RNA stored on FTA paper incubated at elevated temperatures 1

From: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting

Influenza B RNA concentration (copies/microliter) Duration of incubation (hours) Incubation temperatures (°C)
24 72 240
1 2 3 1 2 3 1 2 3
106 + + + + + + + + + 20-25 (RT)
  + + + + + + + + + 30
  + + + + + + + + + 30-45 (outdoors)
104 + + + + + + + + + 20-25 (RT)
  + + + F + + + + + 30
  + + + + + + + + + 30-45 (outdoors)
102 + + F F F 20-25 (RT)
  F F 30
  F F F F F 30-45 (outdoors)
106 (control RNA) +    +    +    −20 (freezer)
102 (control RNA) +    +    +    −20 (freezer)
  1. 1Each experiment for particular combination of sample concentration and temperature was run in triplicate. Plus sign indicates that RT-PCR detection produced strong band of expected size that was observed on a gel; F indicates a faint band and a minus sign indicates absence of an amplification product detectable by visual inspection on a gel.