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Table 1 Laboratory design and main equipment in BSL-3 laboratory according to WHO biosafety guidelines

From: Feasibility of establishing a biosafety level 3 tuberculosis culture laboratory of acceptable quality standards in a resource-limited setting: an experience from Uganda

Item Biosafety level 3
  WHO biosafety manual Current laboratory Description
Isolationa of laboratory Yes Yes On the topmost level of the building with highly restricted access
Room sealable for decontamination Yes Yes All windows are sealed and checked for leakages
Ventilation    
Inward airflow Yes Yes Inward airflow and ventilation system is automatically monitored with auto-sensors and two Magnehelic® gauges (for supply and exhaust each) and re-validated and certified annually by Air-filter maintenance services (AFMS) group of South Africa
Controlled ventilating system Yes  
HEPA-filtered air exhaust Yes/Nob
Double-door entry Yes Yes Doors are well sealed
Airlock No No  
Airlock with shower No No  
Anteroom Yes Yes Anteroom is under negative pressure
Anteroom with shower Yes/Noc No  
Effluent treatment Yes/Noc No All effluent is decontaminated in the BSL-3 laboratory before is discharged to join the sewage system of the National Referral Hospital (Mulago)
Autoclave    Inside the containment section of the laboratory
On site Yes Yes
In laboratory room Desirable Yes
Double-ended Desirable No
Biological safety cabinets Yes Yes Well serviced by AFMS annually
Personnel safety monitoring capabilityd Desirable Yes Reception and the laboratory sections are connected to intercom system and CCTV camera with a link to the laboratory manager and director’s offices
  1. aEnvironmental and functional isolation from general traffic.
  2. bDependent on location of exhaust.
  3. cDependent on agent(s) used in the laboratory.
  4. dFor example, window, closed-circuit television, two-way communication.